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. Author manuscript; available in PMC: 2014 Apr 21.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Jul 25;35(12):2139–2151. doi: 10.1111/j.1530-0277.2011.01569.x

Figure 6.

Figure 6

Effects of chronic ethanol and NAC treatment on upstream mediators of insulin/IGF signaling. Liver protein homogenates were used to measure immunoreactivity corresponding to (A) insulin receptor (R), (B) IGF-1R, (C) IRS-1, (D) pYpY1162/1163-IR, (E) pYpY1135/1136-IGF-1R, (F) pS312-IRS-1 with a bead-based Multiplex ELISA platform (see Methods and Methods). (G–I) In addition, the phosphorylated/total protein ratios for were calculated to assess relative levels of phosphorylation of each protein. Data were analyzed statistically using one-way ANOVA with the Tukey multiple comparison tests. Significant P-values are indicated within the panels.