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. Author manuscript; available in PMC: 2014 Apr 21.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Jul 25;35(12):2139–2151. doi: 10.1111/j.1530-0277.2011.01569.x

Figure 7.

Figure 7

Effects of chronic ethanol, and NAC treatment on the downstream mediators of insulin/IGF signaling. Liver protein homogenates were used in bead-based Multiplex ELISAs to measure (A) Akt, (B) GSK-3β, (C) p70S6K, (D) PRAS40, (E) pS473-Akt, (F) pS9-GSK3β, (G) pTpS421/424-p70S6K and (H) pT246-PRAS40 immunoreactivities. (I–L) In addition, the phosphorylated/total protein ratios for were calculated to assess relative levels of phosphorylation of each protein. Data were analyzed statistically using one-way ANOVA with the Tukey multiple comparison tests. Significant P-values are indicated within the panels.