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. 2014 Apr 21;9(4):e94873. doi: 10.1371/journal.pone.0094873

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Xenopus embryos were injected with 400LacZ, Xenopus march8 or zebrafish march8 mRNA. Animal caps were dissected and incubated as described in Materials and Methods. Caps were photographed at the indicated times after being placed in dissociation media.