Figure 7. E-cadherin is ubiquitinated by March 8.

HEK293T cells were transfected with HA-Ubiquitin, E-cadherin-GFP, and WT or W109A mutant March8, as indicated, and March8 expression was induced with tebufenozide (see Materials and Methods). E-cadherin was immunoprecipitated (IP) from lysates and immunoblotted (IB) with anti-HA antibody to detect ubiquitinated E-cadherin. Expression of the different proteins was checked by IB of lysates. Three additional independent experiments, and quantification of E-cadherin ubiquitination, are shown in Figure S5.