Figure 6. Nuc2 activity is detectable in mouse infections.

A. Activation of the TT probe by recombinant micrococcal nuclease (left) or spent culture media (orange) or cell suspensions (green) of S. aureus WT, a strain only expressing Nuc2 (nuc mutant), and strain without nucleases (nuc nuc2 double mutant). B. TT probe activation at infection sites of leg muscle tissue of mice bearing infections with the same three strains. This image is representative of 3 independent experiments.