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. 2014 Apr 21;9(4):e95694. doi: 10.1371/journal.pone.0095694

Figure 3. SH-11052 blocks DNA synthesis in endothelial cells.

Figure 3

After treatment with the indicated concentrations of SH-11052 (2) and an EdU pulse, the HUVECs (A, B) and HRECs (C, D) were stained with DAPI (for nucleus - blue) and incorporated EdU (in proliferating cells – red) using a Click-iT kit (Life Technologies). The cells were counted from 6 different fields of a coverslip and the percentage of proliferating HUVECs (B) and HRECs (D) was calculated from the ratio of EdU stained cells to DAPI stained cells in each section (dots in the graphs) using ImageJ analysis software. The lines indicate the mean ± SEM and *** indicates p<0.0001 (ANOVA with Dunnett's post hoc test). Representative data from three independent experiments.