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. 2014 Apr 21;9(4):e95694. doi: 10.1371/journal.pone.0095694

Figure 4. SH-11052 inhibits in vitro angiogenesis without causing apoptosis.

Figure 4

(A) Tube formation on Matrigel by HRECs in the presence of the indicated concentrations of 2. (B) Polygons formed (open spaces) were counted. Mean ± SEM of n = 3 wells. *, p<0.05; ***, p<0.001 compared to DMSO control (ANOVA with Dunnett's post hoc test). (C) HRECs were treated with indicated concentrations of 2 or staurosporine (SP) and stained with DAPI (for nucleus - blue) and activated caspase-3 antibody (red). (D). Percentage of HRECs undergoing apoptosis was calculated by counting number of caspase (red) stained cells compared to total (blue) cells using ImageJ software. Mean ± SEM of cells from 3 different sections; representative data from two independent experiments. *** p<0.001 compared to DMSO control (ANOVA with Dunnett's post hoc test).