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. 2014 Apr 21;9(4):e95694. doi: 10.1371/journal.pone.0095694

Figure 5. SH-11052 inhibits TNF-α mediated NF-κB signaling.

Figure 5

(A) After treating HRECs with the indicated concentrations of 2, p65 (green) was detected by immunofluorescence and nuclei (blue) stained with DAPI. Representative data from three independent experiments. (B) The protein levels of IκB-α were measured after TNF-α treatment in the presence of the indicated concentrations of compound 2 by immunoblot. (D) The phosphorylation level of p38 MAPK in HRECs stimulated with TNF-α was monitored in the presence of the indicated concentrations SH-11052 by immunoblot. (C & E) Densitometry was performed using Quantity One software and analyzed using GraphPad Prism. The lines indicate the mean ± SEM of three biological replicates and * indicates p<0.05 compared to TNF-α treatment (ANOVA with Dunnett's post hoc test.