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. 2014 Apr 21;9(4):e95694. doi: 10.1371/journal.pone.0095694

Figure 6. SH-11052 decreases the expression of NF-κB target genes.

Figure 6

(A) Endothelial activation marker VCAM-1 (red) was detected by immunofluorescence in HRECs exposed to TNF-α ± 2. (B) MetaMorph fluorescence intensity analysis of VCAM-1 staining in the presence of TNF-α and the indicated concentrations of 2, mean±SEM of n = 5 fields; *, p<0.05 **, p<0.01 compared to DMSO control (ANOVA with Dunnett's post hoc test); representative data from two independent experiments. (C) qRT-PCR using TaqMan probes showed that mRNA levels of NF-κB target genes IL8 (interleukin-8) (left panel), CCL2 (MCP-1) and PTGS2 (COX2) (right panel), all induced by TNF-α, were decreased in the presence of 2 in a dose dependent manner. Note different y-axis scales. Mean ± SEM of n = 3 replicates shown; representative data from two independent experiments.