Table 2. Types of KoRV polymorphisms detected across 6 museum specimens of koalas.
LTRs | gag leader | gag | pol | env | Total | |||||||
Sequence length | 505 bp | 465 bp | 1566 bp | 3384 bp | 1980 | |||||||
Shared | Private | Shared | Private | Shared | Private | Shared | Private | Shared | Private | Shared | Private | |
Indels | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Polymorphisms of which: | 17 | 14 | 9 | 4 | 17 | 12 | 19 | 19 | 12 | 13 | 74 | 64 |
Non-coding | 17 | 14 | 9 | 4 | NA | NA | NA | NA | NA | NA | 26 | 18 |
Coding region of which | NA | NA | NA | NA | 17 | 12 | 19 | 19 | 12 | 13 | 48 | 44 |
Stop codon | NA | NA | NA | NA | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 |
Synonymous | NA | NA | NA | NA | 8 | 5 | 9 | 7 | 3 | 3 | 20 | 15 |
Non-synonymous of which: | NA | NA | NA | NA | 9 | 7 | 10 | 11 | 8 | 9 | 27 | 27 |
Not radical | NA | NA | NA | NA | 7 | 3 | 5 | 8 | 2 | 3 | 14 | 14 |
Radical | NA | NA | NA | NA | 2 | 4 | 5 | 3 | 6 | 6 | 13 | 13 |
LTRs are the long terminal repeats, sequence length is from the KoRV reference sequence AF151794. Private polymorphisms were those detected in one koala, shared polymorphisms in more than one koala; NA is not applicable. Radical changes are atypical amino acid substitutions with negative scores in both BLOSUM62 and BLOSUM90 matrices. Radical mutations in env (boldface) were more common (pā=ā0.040) than in gag-pol, relative to other amino acid changes.