Table 1. Primers used in real-time PCR [14].

Gene	GenBank Accession No.	Forward Primer	Reverse Primer	Amplicon Size (bp)
8S rRNA	BK000964	GAGGTTCGAAGACGATCAGA	TCGCTCCACCAACTAAGAAC	315
Scnn1a	NM_011324	AACGACCAAACGAACCFAACAC	GCTCAGAAGGCACACAAGAAGG	315
Scnn1b	NM_011325	CTCGGTGCTGTGCCTCATTG	GCCTCAGGGAGTCATAGTTGGG	278
Scnn1g	NM_011326	TGGTCCTCCTATCCTCGTTCTG	GTCACACCCATCAGGCAATAGC	344

18S rRNA, 18S rRNA; Scnn1a, b, and g, Na⁺ channel, nonvoltage-gated, type I, α, β, and γ (epithelial Na⁺ channel α, β, and γ).