Table 2. Primers used for coding sequence amplification and qRT-PCR analysis.
TIGR gene ID | Primer sequence (5′→3′) | |
Forward | Reverse | |
LOC_Os05g01700 | TGAGTCTGCGCTTCAAGAAA | TATGCTCATCCCTGCAACTC |
TAGCTGCAGTTGCAGAAGGA | TTCGAGTTGCTGTTCTGTGG | |
LOC_Os05g01710 | TCTGGAATTTGCTCGCGTTC | AAACCCTGACCTCGCAGTTA |
CTATGACGGAAGCCTTGGAG | CAGGCCACAATCTTCACCTT | |
LOC_Os05g01730 | TAGTGAGTGGTGAGAGGGGA | AAAAGCCTACCCAAACTGCC |
GCCCCTTCTGCTACATTGAG | TGTGAGTGCTGAACCCTGAA | |
Actin | GATTGCCAAGGCTGAGTACGA | AAAAGAGAGAAACAAGCAGGAGGA |
Note: For each putative gene, primers for coding sequence amplification are shown in the upper row; primers for qRT-PCR are shown in the lower row. The primers for Actin gene were used for qRT-PCR.