(A) In vitro toxicity. Monolayer cultured ovarian SKOV (diamond), breast MCF7 (circle), and prostate PC3 (triangle) cells were treated with PCat (solid line) or DC (dashed line) liposomes for 6 hr, and cell viability was measured at 72 hr after treatment using MTT assay (n=4). (B) Spleen weight. Immunocompetent CD1 mice were given intravenous injections of physiological saline, PCat-siSurvivin or DC-siSurvivin (n=5 per group). The siSurvivin dose was 1 nmole and the DOTAP dose was 0.12 mg, per injection. Spleen was removed 7 days after treatment and weighed. (C) Liver functions. Animals were treated as above. Blood samples were obtained via cardiac puncture and liver function tests (i.e., alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels) were performed.