Skip to main content
. 2013 Sep 25;1(3):267–281. doi: 10.4248/BR201303005

Figure 6.

Figure 6

AAV-sh-Rgs10 reduced the expression of osteoclast marker genes and cytokines in periodontal lesions. (A) qPCR of osteoclast marker genes (i.e., Cathepsin K), genes important for osteoclast differentiation (i.e., RANKL and NFATC1) and cytokines (i.e., IL-1α, and IL-17A) in the periodontal lesion from uninfected mice (Normal) or infected mice treated with AAV-sh-Luc-YFP or with AAV-sh-Rgs10. Hprt was used as an endogenous control. AAV-sh-Rgs10 reduced the expression of inflammatory cytokines in the periodontal tissues (pooled 3 samples each time in each group on three independent experiments). (B) ELISA experiment for inflammatory cytokine expression from osteoclast or T-cells in the periodontal tissues of uninfected mice (Normal) or W50-infected mice treated with AAV-sh-luc-YFP or with AAV-sh-Rgs10. The data of ELISA indicates that uninfected mice (Normal) and W50-infected mice treated with AAV-sh-Rgs10 have less inflammatory cytokines expression of IL-6, IL-1α, IL-17 and TNF-α, compared to W50-infected mice treated with AAV-sh-luc-YFP (pooled 3 samples each time in each group on three independent experiments). N.S: no significant; *P<0.05; **P<0.01; ***P <0.001.