Figure 1. Establishment of the FcγR-expressing reporter cell lines.

(A) The cell surface expressions of FcγRs in Jurkat/FcγR cells were analyzed by flow cytometric analysis. (B) The crosslinking of FcγRs by anti-FcγR monoclonal antibodies induced the tyrosine-phosphorylated proteins in Jurkat/FcγR cells. (C) Bridging between calcein-labeled Daudi and calcein-violet-labeled Jurkat/FcγR cells via rituximab was analyzed by flow cytometry. (D) The crosslinking of FcγRs by anti-FcγR monoclonal antibodies induced the luciferase activities in Jurkat/FcγR/NFAT-Luc cells. The assays were performed in triplicate, and the data are the mean ± SEM.