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. 2014 Apr 21;9(4):e95784. doi: 10.1371/journal.pone.0095784

Figure 1. Cebpa knockdown increases murine marrow cell monopoiesis relative to granulopoiesis and generates a blast population.

Figure 1

A) Diagram of marrow transduction protocol. B) Western blot for C/EBPα and β-actin after transduction and puromycin selection with the pLKO.1 vector (Vec) or with Cebpa shRNAs B9 or B11. C) Number of CFU-M, CFU-G, or CFU-GM per 1E4 cells plated in methylcellulose culture with IL-3, IL-6, and SCF (n = 6–9). D) FACS analysis for Mac-1 and Gr-1 on pooled CFUs. Arrows indicate immature, lineage-negative cells (top). Morphology of pooled CFU cells (bottom). *p<0.05, **p<0.01, ***p<0.001.