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. 2014 Apr 21;9(4):e95784. doi: 10.1371/journal.pone.0095784

Figure 3. Cebpa knockdown increases myeloid colony replating.

Figure 3

A) Myeloid CFUs obtained after transduction with the pLKO.1 vector or with Cebpa shRNAs B9 or B11 were replated each 7 days. The average number of generations for which CFUs were obtained is plotted (top, n = 4–5). G4 indicates that initial CFUs could be replated 3 times to yield CFUs. CFU-G and CFU-M obtained at each generation from a typical experiment done in triplicate, 1E4 cell/plate (bottom). B) FACS analysis for Mac-1 and Gr-1 from G3 CFUs. Arrows indicate immature, lineage-negative cells (top). Morphology of G4 and G6 CFUs from the same experiment (bottom).