Figure 9. Myeloid CFUs and RNAs expression in Lin⁻Sca-1⁻c-Kit⁺ murine marrow cells expressing G-CSF versus M-CSF receptor.

A) FACS analyses demonstrating isolation of Lin⁻Sca-1⁻c-Kit⁺ GCSFR(GR)⁺MCSFR(MR)⁻ and Lin⁻Sca-1⁻c-Kit⁺GCSFR(GR)⁻MCSFR(MR)⁺ populations. B) The number of CFU-M, CFU-G, or CFU-GM obtained per 1E4 cells plated in methylcellulose culture with IL-3, IL-6, and SCF is shown (n = 3). C) Total cellular RNAs were subjected to quantitative RT-PCR for indicated mRNAs (n = 3).