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. 2014 Apr 21;9(4):e95771. doi: 10.1371/journal.pone.0095771

Figure 4. Acting via SIK2, adducin controls the expression of transcription factors and gene expression associated with CH.

Figure 4

A) Presence of SIK1 and SIK2 isoforms in HL-1 cells in culture. An equal amount of protein (150 µg) was loaded in each condition. SDS-PAGE and Western blot was performed and a representative blot is shown. B) SIK2 mRNA expression levels in HL-1 cells expressing either the normotensive (NT) or hypertensive (HT) variant of α-adducin. C) SIK2 activity in HL-1 cells expressing either the normotensive (NT) or hypertensive (HT) variant of α-adducin. Bars represent the mean + s.e.m. of 6 experiments. D) β-myosin heavy chain (β-MHC) luciferase activity in HL-1 cells co-expressing either variant (NT or HT) of α-adducin with the SIK2 wild-type or a negative mutant (SIK2-K49M). E) Brain natriuretic peptide (BNP) luciferase activity in HL-1 cells expressing co-expressing either variant (NT or HT) of α-adducin with the SIK2 wild-type or a negative mutant (SIK2-K49M). F) MEF2C luciferase activity in HL-1 cells co-expressing either variant (NT or HT) of α-adducin with the SIK2 wild-type or a negative mutant (SIK2-K49M). Mean + s.e.m. of 6 experiments performed in duplicate are shown.