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. 2014 Apr 21;9(4):e95977. doi: 10.1371/journal.pone.0095977

Figure 3. Analysis of immunoproteasome protein expression during T. cruzi infection.

Figure 3

(A) Lysates (25–50 µg) of HeLa cells treated with IFN-γ and/or infected with T. cruzi were analyzed by western blot using anti-immunoproteasome subunits antibodies as indicated. (B) Protein levels were determined by densitometry and plotted using the expression of α6 subunit as experimental normalizer. Infection was confirmed using anti-tubulin antibody. (C) Western blot analysis of PA28β expression during different times of infection. HeLa cells were pre-treated with IFN-γ for 24 h and then infected with T. cruzi for 24, 48 and 72 h. (D) Protein levels were plotted using the expression of α1 as experimental normalizer. All values in this figure represent mean ± standard deviation of three individual experiments.