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. 2014 Apr 21;9(4):e95977. doi: 10.1371/journal.pone.0095977

Figure 6. Effect of T. cruzi infection on MHC class I cell surface expression.

Figure 6

(A) Immunofluorescence microscopy of HeLa cells treated with IFN-γ and/or T. cruzi-infected stained with human anti-MHC class I antibody (yellow) and DAPI (blue). The arrows highlight the infected cells that clearly had the MHC class I expression down-modulated by T. cruzi infection. Bars  = 25 µm. To precisely quantify the MHC class I cell surface expression, samples used in microscopy were analyzed by flow cytometry. (B) The protein expression was determined as median fluorescence intensity (MFI). Each value represents the mean ± standard deviation of three independent experiments.