Table 1. The absorption, emission, quantum yield, distinctive coefficient, and relative brightness values of all the FPs and iq-FPs.

	Abs max (nm)	Em max (nm)	QY	ε (cm−1 M−1)	relative brightness
EBFP2	386	447	0.57	22,300	0.34
iq-EBFP2	386	446	0.63	24,800	0.42
mCerulean3	432	475	0.99	27,300	0.72
iq-mCerulean3	433	474	0.92	27,700	0.68
mEmerald	482	509	0.74	51,200	1.00
iq-mEmerald	488	511	0.67	50,400	0.89
mVenus	516	529	0.64	94,800	1.62
iq-mVenus	516	529	0.63	88,700	1.48
mApple	569	593	0.37	43,900	0.43
iq-mApple	568	593	0.36	34,400	0.33
mKate2	588	629	0.38	37,600	0.38
iq-mKate2	580	632	0.34	11,600	0.11

The detailed measurement protocol is described in Materials and Methods section. Relative brightness (QY * ε) is normalized with respect to mEmerald.