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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Oncogene. 2013 Oct 21;33(42):4997–5005. doi: 10.1038/onc.2013.437

Figure 3.

Figure 3

cPf/f78f/f livers exhibited increased proliferation, progenitor cell expansion and GRP78 expression in bile duct cells. (a) Immunohistochemistry staining of cell proliferation marker Ki67 (brown nuclei) on livers of the indicated genotypes at 3 and 6 months. (b) Real-time quantitative PCR analysis of liver progenitor markers EpCAM, AFP and CK19 in livers of the indicated genotypes at 3 months. Expression levels of each gene were normalized to 18S RNA levels. Data are presented as mean ± S.E. *p<0.05. (c) Immunohistochemistry staining of GRP78 on livers from the indicated genotypes aged 3 and 6 months. Far right panels represent higher magnification of the boxed regions in cPf/f78f/f livers. Black arrowheads in panels a and c denote examples of bile ducts. Scale bars show 100 µm and are applicable to all panels.