Table 2.
Detection of SBV RNA in IFNAR-/- mice inoculated with 100 μL of semen from bull 7 (Experiments 1 and 2) or with SBV RNA positive blood from IFNAR-/- mice (Experiment 3)
| Inoculum | Exp. N° | Mice N° | C t inoculum | C t blood (4 days pi) | ELISA results |
|---|---|---|---|---|---|
| Bull 7 (24/08/2012) |
2 |
8587 |
33.6 |
36.7 |
Neg |
| 8585 |
Neg |
Neg |
|||
| 8589 |
24.0 |
Neg |
|||
| 8581 |
Neg |
Neg |
|||
| Bull 7 (29/08/2012) |
1 |
7728 |
17.1 |
28.9 |
Pos |
| 8216 |
23.5 |
Pos |
|||
| 8410 |
23.0 |
Pos |
|||
| 2 |
8583 |
19.7 |
21.7 |
Pos |
|
| 8849 |
20.0 |
Pos |
|||
| 8582 |
25.6 |
Pos |
|||
| 8588 |
26.0 |
Pos |
|||
| Bull 7 (07/09/2012) |
2 |
8827 |
22.3 |
31.5 |
Pos |
| 8571 |
Neg |
Neg |
|||
| 8847 |
Neg |
Neg |
|||
| 8856 |
37.0 |
Pos |
|||
| Bull 7 (17/09/2012) |
2 |
8621 |
23.1 |
Neg |
Neg |
| 8709 |
Neg |
Neg |
|||
| 8853 |
Neg |
Neg |
|||
| 8630 |
Neg |
Neg |
|||
| Inoculum A (1/10) |
3 |
8631 |
22.0 |
22.1 |
Pos |
| 8634 |
Neg |
Neg |
|||
| 8858 |
Neg |
Neg |
|||
| 8629 |
Neg |
Neg |
|||
| Inoculum A (1/100) | 3 | 8688 |
25.0 | Neg |
Neg |
| 8682 |
Neg |
Neg |
|||
| 8848 |
Neg |
Neg |
|||
| 8625 | Neg | Neg |
qRT-PCR results from 10 μL of straws or blood sampled from IFNAR-/- mice, 4 days pi.
Bold data correspond to samples with a confirmed detection of SBV RNA (Ct inoculum and blood) or positive ELISA results.