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. 2014 Apr 1;22(7):2320–2326. doi: 10.1016/j.bmc.2014.02.004

Figure 2.

Figure 2

Side product after incomplete deprotection of amino-on CPG. The recommended reaction time for oligonucleotide resin cleavage of 10 min in AMA at 65 °C proved to be insufficient for complete deprotection of the amino function of the amino-on CPG. The nitrophenyl amide was found to be still partially attached. A second deprotection step of the mixture in concentrated ammonia for 4 h provided a homogenous solution of the oligonucleotide with free primary amine. Alternatively the amino-on CPG can be completely deprotected by longer incubation in AMA.