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. 2014 Apr 1;22(7):2320–2326. doi: 10.1016/j.bmc.2014.02.004

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

HPLC of PEGylated antisense strand (5) after purification. Semi-preparative reversed phase HPLC with a linear gradient of acetonitrile (8–30% in 30 min) in TEAA buffer was used for separation of PEGylated siRNA oligonucleotides 5 and 6 from unreacted educts (3 and 4) and NHS-PEG₁₂. Analytical HPLC of the respective fractions with the same conditions confirmed the successful purification and high purity of the isolated compound.