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. 2014 Apr 1;22(7):2320–2326. doi: 10.1016/j.bmc.2014.02.004

Figure 4.

Figure 4

Gene knockdown in a luciferase reporter assay after co-transfection of psiCHECK2-bcl-2 plasmid and siRNA. A psiCHECK2 plasmid with the cDNA of the human bcl-2 gene fused to the Renilla luciferase gene was transfected together with the indicated siRNA into MCF-7 breast cancer cells. Bcl-2 targeted gene knockdown reduces the signal of the Renilla luciferase, while the Firefly luciferase is used for normalization of transfection efficiency and cell number. Knockdown effects are reported in normalized Renilla luciferase intensities (n = 3), error bars represent the standard deviation.