Table 3.
Intracellular cobalt solubilization assessed by single-cell quantitative imaging of cobalt (micro-PIXE) after exposure of BEAS-2B cells to Co 3 O 4 particles for 72 h
| Co mean ± SD (fg/cell) | IC25 | IC50 |
|---|---|---|
| Particulate Co |
9500 ± 1650 |
20,450 ± 23,500 |
| min 6593 – max 11,483 |
min 3062 – max 78,486 |
|
| Solubilized Co |
6.7 ± 3.0 |
48 ± 20 |
| min 3.4 – max 11.5 |
min 12.0 - max 70.0 |
|
| % solubilization | 0.07 ± 0.03 |
0.55 ± 0.50 |
| min 0.04% - max 0.12% | min 0.06% - max 1.6% |
Mean ± SD for the particulate and solubilized intracellular fractions (n = 6 for IC25 and n = 9 for IC50). The solubilization percentage (mean ± SD) was also calculated from each single-cell analysis, and not from the mean solubilized to particulate ratios. Minimal and maximal values are indicated below the mean values.