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. 2014 Apr 3;18(2):121–127. doi: 10.4196/kjpp.2014.18.2.121

Fig. 5.

Fig. 5

Number of c-Fos-positive neurons at different groups in laminae I-II and laminae III-VI on the L4 and L5 segments of the spinal cord ipsilateral to the site of formalin injection. Experimental groups were classified as follows: normal (no treatment group); formalin-only (formalin-injection-only group); ST36-For (manual acupuncture treatment at ST36 before formalin injection); SP9-For (manual acupuncture treatment at SP9 before formalin injection); BL60-For (manual acupuncture treatment at BL60 before formalin injection); and NA-For (manual acupuncture treatment at a non-acupoint before formalin injection). ST36, SP9, BL60, and non-acupoint groups showed significantly decreased numbers of c-Fos-positive neurons in laminae I-II. The BL60 group in particular had significantly reduced c-Fos-positive neurons in laminae III-VI. The total number of c-Fos-positive neurons from laminae I to VI showed significantly inhibited c-Fos immunoreactivity in the ST36, SP9 and BL60 groups. Each bar represents the group mean±SEM (*p<0.05, **p<0.01 for comparison between the normal group and all other groups, one-way ANOVA followed by Dunnett's post-hoc multiple comparison. #p<0.05, ##p<0.01 for comparison between the formalin-only group and the ST36-For, SP9-For, BL60-For, and NA-For groups, one-way ANOVA followed by Dunnett's post-hoc multiple comparison).