Fig. 4.

The effect of [6]-shogaol, [6]-gingerol, and zingerone on 5-HT concentration-response curves. (A) Representative currents traces activated by 1 mM 5-HT in the presence or absence of [6]-shogaol (10 µM), [6]-gingerol (30 µM), and zingerone (1 mM). Various concentrations of reagents were pretreated 30 sec, and then co-application with 5-HT. (B) Concentration-response relationship for 5-HT in the absence and presence of 10 µM [6]-shogaol, 30 µM [6]-gingerol, and 1 mM zingerone. The current amplitude is normalized to the current activated by 1 mM 5-HT. Data points are the mean±S.E.M. (n=4~6). The curve is the best fit of the data to the Hill equation described in methods.