Table 3.
Polymorphism in the fragment of 16S rRNA gene in A. phagocytophilum isolates from ticks and human pathogenic strains (sequences published in GenBank)
| Strain/genetic variant |
Nucleotide positions 5’ → 3’
a
|
Quality of habitat/country | No. of isolates (reference no. of samples)/site of study | GenBank acc. number | Host/vector found in other studies (only 100% homology; nucleotide sequences deposited in GenBank) | ||||
|---|---|---|---|---|---|---|---|---|---|
| 199 | 328 | 376 | 539 | ||||||
| This study |
I |
G |
A |
G |
G |
natural/ Poland |
2 (6,153)/KNP |
KF312352 |
bank vole (Myodes glareolus, KC740432); dog (KF985242); elk (Alces alces, KC800983); roe deer (Capreolus capreolus, JQ965530); cotton rat (Sigmodon hispidus, JQ063025); rat (Rattus norvegicus, KC470064); reindeer (Rangifer tarandus, JX841254); hedgehog (Erinaceus europaeus, JN571163); I. ricinus (KF481930), I. trianguliceps (KF481934) |
|
6 (39, 54, 73, 85, 90, 283)/BNP | |||||||||
| II |
G |
A |
A |
G |
urban/Poland |
8 (29, 37, 38, 47, 62, 67, 71, 73)/Bielański Forest |
KF312353 |
dog (EF668225); cat (HM138366); I. ricinus (JX909354); D. reticulatus (KF381413) |
|
| III |
T |
A |
A |
G |
1 (57)/Bielański Forest |
KF312351 |
n. m.b |
||
| IV |
G |
A |
A |
T |
1 (64)/Bielański Forest |
KF312354 |
n. m.b |
||
| A. phagocytophilum strains pathogenic for human | G |
A |
G |
G |
Poland |
KF111754 |
|||
| G |
A |
G |
G |
Slovenia |
GU236658 |
||||
| G |
A |
G |
- |
Italy |
DQ029028 |
||||
| G |
A |
G |
G |
USA |
GU236664 |
||||
| G | A | G | G | USA | NR_074113 | ||||
aThe number corresponds to the positions of nucleotide substitutions relative to the sequence of the complete 16S rRNA gene of A. phagocytophilum strain HZ (NR_074113). Base substitutions are shown in bold.
bNo match has been found in GenBank.