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. 2014 Mar 21;7:109. doi: 10.1186/1756-3305-7-109

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Copyright © 2014 Scharsack and Kalbe; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Respiratory burst activity in D. pseudospathaceum exposed sticklebacks. Head kidney leukocytes were stimulated with zymosan (A) and D. pseudospathaceum antigens (B). Respiratory burst activity was recorded with a chemiluminescence assay (RLUarea) 1.5 and 5 days after the last exposure (mean and SE, 0/0 = sham exposed controls, 0/1 = single exposure only, 5/0 = pre-exposures only, 5/1 = pre- and single exposures, different letters above bars indicate significant differences p < 0.05 with sequential Bonferroni correction for multiple testing). Note: different y-axis scaling in A and B.