Table 2.
Main effects of statistical models of the immune parameters of D. pseudospathaceum exposed sticklebacks
| |
|
RLUarea zymosan |
RLUarea
D. p.
antigens |
% S + G
2-m
lymphocytes |
% S + G
2-m
monocytes |
||||
|---|---|---|---|---|---|---|---|---|---|
| df | χ 2 | P value | χ 2 | P value | χ 2 | P value | χ 2 | P value | |
| Intercept |
1 |
882.0 |
< 0.001 |
750.1 |
< 0.001 |
1880.4 |
< 0.001 |
2249.9 |
< 0.001 |
| Family(origin) |
6 |
49.8 |
< 0.001 |
45.4 |
< 0.001 |
8.1 |
0.232 |
9.6 |
0.145 |
| Sampling time point |
1 |
0.5 |
0.501 |
18.6 |
< 0.001 |
4.9 |
0.028 |
0.2 |
0.632 |
| Origin |
1 |
20.6 |
< 0.001 |
23.7 |
< 0.001 |
1.5 |
0.220 |
1.1 |
0.291 |
| Exposure treatment |
3 |
2.9 |
0.401 |
0.98 |
0.804 |
10.7 |
0.013 |
7.2 |
0.065 |
| Samp. t. x orig. x exp. treat. | 10 | 23.4 | 0.009 | 7.2 | 0.705 | 7.1 | 0.716 | 41.8 | < 0.001 |
Respiratory burst activity (RLUarea) was recorded with zymosan and stimulation with D. pseudospathaceum antigens (Figure 4), and proliferation (% S + G2-m) of lymphocytes and monocytes (Figure 5) was recorded from n = 446 sticklebacks (Figure 1). Data were analysed with Generalized Linear Models (GzLM) with stickleback family nested in stickleback origin (lake/river).