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. 2014 Mar 21;13:112. doi: 10.1186/1475-2875-13-112

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Copyright © 2014 Mata-Cantero et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Establishment of the initial parasitaemia after magnetic column purification. Plasmodium falciparum iRBCs isolated with magnetic columns were adjusted to 0.2% haematocrit and different parasitaemias (1, 5, 10, 15, and 20%). Plasmodium falciparum cultures were harvested after 18 hours to determine the parasitaemia levels. A. Giemsa-staining smears from cultures purified by magnetic purification and allowed to reinvade new uRBCs. B. Parasitaemia achieved at 0.2% haematocrit with the different initial parasitaemias tested was estimated from blood-smears (n = 3). C. Invasion rate calculated accounting for the parasitaemia obtained after 18 hours (final parasitaemia) and the initial parasitaemia of the P. falciparum culture (final parasitaemia/initial parasitaemia). This rate indicates the capability of the parasites to invade new RBCs.