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. 2014 Apr 22;4:4732. doi: 10.1038/srep04732

Figure 3. LIEGS suppressed the IL-2 expression by blocking JNK signaling.

Figure 3

LIEGS suppressed the calcium ionophore and phorbol ester-induced IL-2 expression levels in Jurkat cells in a concentration-dependent manner (a). Differences compared with the control were analyzed by Student's t-test. *p < 0.05, **p < 0.01. Phosphorylation of JNK was analyzed by Western blotting. Stimulated Jurkat cells were lysed and total protein concentrations were determined from cellular extracts. Equal amounts of total proteins (60–80 μg) were separated by 10% SDS-PAGE and transferred onto PVDF membranes for the detection of each IL-2 signal transduction proteins using phosphorylated protein-specific antibodies. After 30-min stimulation by calcium ionophore and phorbol ester, the phosphorylation of JNK was significantly increased, and this phosphorylation was blocked by the simultaneous treatment with LIEGS. Representative Western blots are shown to (b). Full-length blots are presented in Supplementary Figure 1.