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. 2014 Apr 22;4:4745. doi: 10.1038/srep04745

Figure 5. In vitro inflammatory effect of fibroin coated Ti surfaces.

Figure 5

(5A–B) Murine macrophage (Raw 264.1) is cultured with or without osteoblast like cells (MG-63) for 24 hr. Cell supernatant was collected and TNF-α, and IL-1β levels are measured by Quantikine kit (Invitrogen, USA). Product quantity is measured at 450 nm and plotted. (5C–D) Nitric Oxide (NO) level in cell supernatant of 24 and 72 hr are is measured at 450 nm and plotted. Statistical analysis is carried out by Student's T-test. * and ** denote statistically significant difference (p < 0.05) between Ti, Am and Bm respectively. No significant differences in IL-1β and NO production are observed between control and experimental samples.