Figure 5.

DHPG-induced mGluR-LTD in slices from PERK cKO mice is associated with an increase of de novo protein synthesis similar to that in wild-type mice. (A) Representative Western blot of lysates from wild-type (WT) and PERK cKO hippocampal slices treated with puromycin (5 μg/mL, 35 min) and incubated with either ACSF (Cntl) or DHPG (100 μM) for 10 min to measure protein synthesis at baseline levels and in response to DHPG treatment. (B) Cumulative graph with percentages expressed relative to WT control (Cntl), n = 3 mice per genotype, 2–3 slices per condition ([*] P < 0.05, Student's t-test), All data represent mean values ± SEM.