FIG 3 .

TPI is required for growth and survival of M. tuberculosis with single carbon sources. Growth and survival followed by absorbance measurement (A) and CFU assay (B) of WT (squares), Δtpi (circles), and tpi-Comp (triangles) in carbon-defined Sauton’s base medium containing the indicated carbon sources (0.4% glucose, 0.2% glycerol, 0.2% acetate, 0.2% glutamate, or a mix of 0.1% of each of the indicated two carbon sources). Dashed lines represent the limit of detection of the CFU determinations. Data are means ± standard deviations (SD) for triplicate cultures (error bars in panel B are smaller than the symbols). Data are representative of at least two independent experiments except for the growth curves with glutamate, which were performed once.