FIG 7 .

TPI is required to establish infection in mice. Bacterial titers in lungs (A) and spleens (B) of C57BL/6 mice infected with the indicated strains were determined. The dashed line represents the limit of detection (4 CFU/organ). After day 1, no CFU were detected in the lungs or spleens from mice infected with the Δtpi strain except in the lung of one animal at day 56, which contained 18 CFU. Data are means ± SD from 4 mice per group and time point and are representative of two independent experiments. (C) Lung sections stained with hematoxylin and eosin from mice infected with the indicated strains at day 56 postinfection. The boxed areas in the left panels (magnification, ×4) are shown in the right panels (magnification, ×20).