TABLE 2 .
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | φ80dlacZΔM15 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 F− Δ(lacZYA-argF) U169 | 49 |
| S17-1 | thi pro hsdR recA Tra+ | 50 |
| P. aeruginosa strains | ||
| K767 | PAO1 prototroph wild type | 51 |
| K1525 | K767ΔmexXY | 52 |
| K3315 | K767ΔmexY | This study |
| Plasmids | ||
| pEX18Tc | Broad-host-range gene replacement vector; sacB Tcr | 53 |
| pCL8 | pEX18Tc::ΔmexY | This study |
| pCL9 | pEX18Tc::mexY | This study |
| pRK415 | P. aeruginosa-E. coli shuttle cloning vector; Tcr | 54 |
| pCL10 | pRK415::mexYWT | This study |
| pCL11 | pRK415::mexYS16F | This study |
| pCL12 | pRK415::mexYR166C | This study |
| pCL13 | pRK415::mexYR184H | This study |
| pCL14 | pRK415::mexYG216D | This study |
| pCL15 | pRK415::mexYV339M | This study |
| pCL16 | pRK415::mexYP562S | This study |
| pCL17 | pRK415::mexYA960T | This study |
| pCL18 | pRK415::mexYK79A | This study |
| pCL19 | pRK415::mexYD133A | This study |
| pCL20 | pRK415::mexYD133S | This study |
| pCL21 | pRK415::mexYY613A | This study |
a
Tcr, tetracycline resistance; WT, wild type.