TABLE 2 .
Strain or plasmid | Descriptiona | Reference or source |
---|---|---|
E. coli strains | ||
DH5α | φ80dlacZΔM15 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 F− Δ(lacZYA-argF) U169 | 49 |
S17-1 | thi pro hsdR recA Tra+ | 50 |
P. aeruginosa strains | ||
K767 | PAO1 prototroph wild type | 51 |
K1525 | K767ΔmexXY | 52 |
K3315 | K767ΔmexY | This study |
Plasmids | ||
pEX18Tc | Broad-host-range gene replacement vector; sacB Tcr | 53 |
pCL8 | pEX18Tc::ΔmexY | This study |
pCL9 | pEX18Tc::mexY | This study |
pRK415 | P. aeruginosa-E. coli shuttle cloning vector; Tcr | 54 |
pCL10 | pRK415::mexYWT | This study |
pCL11 | pRK415::mexYS16F | This study |
pCL12 | pRK415::mexYR166C | This study |
pCL13 | pRK415::mexYR184H | This study |
pCL14 | pRK415::mexYG216D | This study |
pCL15 | pRK415::mexYV339M | This study |
pCL16 | pRK415::mexYP562S | This study |
pCL17 | pRK415::mexYA960T | This study |
pCL18 | pRK415::mexYK79A | This study |
pCL19 | pRK415::mexYD133A | This study |
pCL20 | pRK415::mexYD133S | This study |
pCL21 | pRK415::mexYY613A | This study |
Tcr, tetracycline resistance; WT, wild type.