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Effect of increasing the goal concentration sf/wf on oocyte volume response during EG addition using two-step (A) and three-step (B) piecewise constant procedures. The horizontal dotted lines show the osmotic tolerance limits. Goal concentration values sf/wf = 30, 35, 37, 50 and 70 correspond with intracellular EG concentrations of 9, 10.5, 11.1, 15, and 21 Osm/kg (in molal units), or approximately 6, 6.6, 6.9, 8.2 and 9.7 mol/L (in molar units).
