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. Author manuscript; available in PMC: 2014 Apr 22.
Published in final edited form as: Methods Mol Biol. 2013;1046:231–250. doi: 10.1007/978-1-62703-538-5_14

Figure 3. Purification of bovine Arp2/3 complex using Superdex 200 gel filtration chromatography.

Figure 3

(A) An example of the UV absorbance detected chromatogram for bovine Arp2/3 complex purification on a 320 mL Superdex 200 pg column. UV absorbance is shown with a thick green line. Fractions collected are shown with short vertical lines. Relevant fraction numbers are shown. (B) SDS-PAGE analysis of relevant fractions was performed a using 15% acrylamide gel, and stained using coomassie brilliant blue dye. At this stage the complex is pure, with no contaminants observed. Multiple isoforms of ArpC1 are present, and appears as a multiplet. Fraction numbers from the chromatogram are indicated at the top of the gel lanes, and molecular weights of standards are shown at the left. Depending on intended use, Arp2/3 complex may be pooled (e.g., fractions 14 – 17) or kept separate.