Skip to main content
. Author manuscript; available in PMC: 2015 Mar 10.
Published in final edited form as: Dev Cell. 2014 Mar 10;28(5):520–533. doi: 10.1016/j.devcel.2014.02.001

Figure 3. Identification of Vac17 residues required for the termination of vacuole movement and Vac17 turnover.

Figure 3

(A) Vac17 residues 204-250 comprise the PEST sequence (Tang et al., 2003). Red; potential phosphorylation sites required for Vac17 turnover. Blue; FHA binding motif. (B) Western blot analysis of vac17 point mutants from an alanine scan of all the serine and threonine residues within the Vac17 PEST sequence. anti-Vac17 antibodies; 1:1,000 dilution. (C) vac17-T240A-GFP and vac17-S222A-GFP as well as vacuoles are mis-targeted to the mother bud neck (arrows). Bar = 5 μm. See also Figure S3.