Figure 2.

Metabolic phenotype of the ASTRO-D2KO mouse kept on regular chow diet at room temperature (22°C). (A) Body weight evolution during the 2-month period. (B) Body weight of the animals right before admission to CLAMS. (C) Body composition measured by DEXA 48 h before the animals were admitted to CLAMS. (D) VO₂ during 12-h light and dark cycles. ASTRO-D2KO mouse and controls were acclimated to individual metabolic cages in the CLAMS for 48 h before measurements were recorded. Data shown are from the second day after acclimatization. (E) Same as in D, except that what is shown is RQ. (F) Contribution of fat oxidation to daily EE during the light cycle. (G) Food intake of the same animals during light and dark cycles. Entries are mean ± SEM of 4–5 animals. Area under the curve was calculated during light and dark cycles for each individual animal. Statistical significance is shown in each graph and was set as P < 0.05. Student t test was used to compare controls and ASTRO-D2KO groups within treatment conditions. Black horizontal bars denote the dark period of the day (12 h). AUC, area under the curve.