Figure 1.

Generation of mice with conditional KO of IG20/MADD in β-cells. A: Shows schematic representation of the strategy used for generating floxed KMA1 mice. B: Shows genotyping results from offspring of KMA1het (KMA1^flox+/−: Cre) mice intercrossing. Primers used for PCR are listed in Supplementary Table 8. Representative figure from 98 mice is shown. C: Results from RT-PCR using RNA isolated from hypothalamus and islets from WT, KMA1het, and KMA1ko mice. Orexin and insulin-2 served as specific markers of hypothalamus and pancreatic islets, respectively. Representative data from 3 mice per group are shown.