Figure 6.

H₂S donor upregulated the expressions of Ang-1 in wound skin tissues and in EPCs in type 2 diabetes, whereas CSE suppression downregulated Ang-1 expression in normal EPCs. Protein was extracted from 6 mm of wound skin and 0.5 cm of skin outside of the wound, and 7-day cultured EPCs after 16 days of in vivo treatment by two H₂S donors and PAG, or normal EPCs with in vitro CSE siRNA 50 nmol/L treatment and its controls. A and B: Protein bands and comparison of EPCs and skin tissue Ang-1 expression in five groups (n = 4–5). *P < 0.01 PAG vs. db/+ control, db/db vs. db/+ control; #P < 0.01 NaHS vs. db/db control and HTB vs. db/db control. C: Protein bands and comparison of VEGF expression of EPCs in five groups (n = 4–5). *P < 0.01 db/db vs. db/+. D: Protein bands and comparison of Ang-1 expression in EPCs with and without CSE siRNA treatment (n = 4–5). *P < 0.01 vs. control.