Figure 7.
H2S promoted EPC angiogenesis by restoration of Ang-1 expression. EPCs were isolated from bone marrow of db/db and normal mice and cultured in EGM-2 for 7 days, then db/db EPCs (5 × 105) were reseeded and treated in vitro with NaHS 50 nmol/L only in EGM-2 for 30 min, or Ang-1 siRNA at 5 nmol/L in medium for 6–7 h before NaHS 50 nmol/L treatment. Then, tube formation on Matrigel and adhesion on vitronectin assay were performed in all EPCs. A: Representative photographs of tube networks under microscope (original magnification ×4). B: Comparison of tube length in four groups of db/+ EPCs and db/db EPCs treated with and without NaHS, NaHS and Ang-1 siRNA (n = 4 in each group). *P < 0.05 vs. db/+, #P < 0.05 vs. db/db only.