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. 2014 Apr 15;4:79. doi: 10.3389/fonc.2014.00079

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Copyright © 2014 Suhovskih, Tsidulko, Kutsenko, Kovner, Aidagulova, Ernberg and Grigorieva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stability of HS-metabolic system upon different external and internal stimuli. (A) Expressions of key HS metabolism-involved genes in LNCaP and PC3 prostate cancer cells before and after the treatments with 5-aza-deoxycytidine (+5-aza-dC), Trichostatin A (+TSA), ectopic over-expression of GLCE (+GLCE), co-culture with human fibroblasts (+fibroblasts). The genes expression levels normalized to that of GAPDH. The graphs show the mean expression levels from triplicate experiments (±s.d.) (OriginPro 8.1). Genes up-regulated or down-regulated >2-fold are shown. (B) Relative activity of HS-metabolic machinery before and after the treatments. Stacked column compares the contribution of each value to a total across categories.