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. 2014 Apr 15;5:174. doi: 10.3389/fimmu.2014.00174

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Copyright © 2014 Haabeth, Tveita, Fauskanger, Schjesvold, Lorvik, Hofgaard, Omholt, Munthe, Dembic, Corthay and Bogen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Matrigel assay. A novel approach to unravel the dynamics of CD4⁺ T cell-mediated primary anti-tumor immune responses. At day 0, subcutaneous injections with MOPC315 tumor cells suspended in liquid Matrigel. When the Matrigel solution reaches body temperature, it jellifies and forms a plug containing the tumor cells. At various time points after injection (n days), the Matrigel plug and tumor-draining lymph nodes are dissected out and analyzed ex vivo for cellular content, function of cells, and cytokines (34, 36, 39, 85).