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. 2014 Apr 14;8:126. doi: 10.3389/fnbeh.2014.00126

Figure 1.

Figure 1

(A) Schematic diagram of the targeting strategy for the novel TMc-Nrg1+/− mutant mice. Upper panel, wild type (WT) Nrg1 locus including 13 exons, shown as black boxes. The target vector, pKOS-53 with a LacZ/Neo cassette, was targeted to exon 9 of Nrg1 by homologous recombination. Lower panel, primer 0932-8, primer 0932-10, and primer Neo3a were used to distinguish WT alleles (346 bps) from mutant alleles (258 bps). (B) Mouse-tail DNA was submitted to PCR analysis. N: negative control. (C) The expression level of Nrg1 proteins in the brain of both WT and Nrg1+/− mice. *P < 0.05 between two genotypes.